Improved protein sample separation using GenScript's SurePAGETM Bis-Tris gels.

Application of the ExperionTM Automated Electrophoresis System to Glycoprotein Visualization and Analysis

Identical amounts of each protein were analyzed by SDSPAGE and with the Experion system (Bio-Rad Laboratories). For SDS-PAGE, samples were prepared by mixing equal volumes of protein sample and 2x Laemmli sample buffer with or without 5% β-mercaptoethanol. Following separation on CriterionTM Tris-HCl 4–20% precast gels, gels were stained with Bio-SafeTM colloidal Coomassie Blue stain and imaged...

Separation of Native Allophycocyanin and R-Phycocyanin from Marine Red Macroalga Polysiphonia urceolata by the Polyacrylamide Gel Electrophoresis Performed in Novel Buffer Systems

Three buffer systems of Imidazole-Acetic acid, HEPES-Imidazole/Bis-tris and Bis-tris-HEPES-MES were designed based on the principle of discontinuous polyacrylamide gel electrophoresis (PAGE) for the native PAGE which could be performed in pH 7.0 and 6.5 in order to analyze and prepare the minor components of allophycocyanin (AP) and R-phycocyanin (R-PC) from marine red macroalga Polysiphonia ur...

Improved electrophoretic separation of polymorphic short tandem repeats in agarose gels using bis-benzimide.

Preparation of protein samples for SDS-polyacrylamide gel electrophoresis: procedures and tips

odium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE) is the most widely used analytical method to resolve separate components of a protein mixture. It is almost obligatory to assess the purity of a protein through an electrophoretic method. SDS-PAGE simultaneously exploits differences in molecular size to resolve proteins differing by as little as 1% in their electrophoretic mobil...

Improved Extraction-Separation of Lanthanum/ Europium Ions by Bis(2-ethylhexyl)phosphoric Acid Using 12-Crown-4 as an Ion Selective Masking Agent

The extraction-separation of lanthanum and europium ions with bis(2-ethylhexyl) phosphoric acid (DEHPA) in cyclohexane was improved by adding 12-crown-4 (12C4) to the aqueous phase as a masking agent. In the presence of this crown ether the extraction curve of lanthanum ions versus pH shifts to the higher pH region, while the curve is not influenced for the europium ions. Consequently the s...